結論

首先，表面張力測量證明牛精漿具有非常好的表面活性，這可能與 BSP 蛋白。 二、朗繆爾薄膜法 先前被證明是相關的脂質單層模型 公牛精子的外細胞膜 [35]，有助于表征 BSP 蛋白對脂質膜的親和力。 BSP 蛋白是 能夠到達被磷脂覆蓋的牛精子細胞表面，這要歸功于它們與磷脂酰膽堿和 它們自身的表面活性。 我們的假設是，由于它們自己的表面，它們首先穿透精子的外葉 活動然后他們留在那里因為與 磷脂酰膽堿。 最后，Langmuir 薄膜法也得到了 用于篩選已知的 BSP 蛋白螯合劑（如 LDL 和脂質體）的作用。 脂質體被證明是 與 LDL 一樣有效地防止 BSP 蛋白插入 磷脂層。 發現 LDL 和 脂質體：0.16–0.17 mg 磷脂酰膽堿/mg BSP。 我們不 還知道脂質體在 冷凍保存的時間尺度有一些生物學后果。 需要進一步的研究來分析每個的表面特性 BSP 蛋白及其對膜的作用。 此外，可以測試朗繆爾薄膜以檢測 BSP 對膜的親和力隨溫度的變化 [20]。 最后，朗繆爾薄膜法是一種 用于鑒定新的螯合劑的強大篩選方法 BSP 蛋白質。 這種方法可以適用于其他動物物種。

致謝

 作者要感謝 IMV Technologies（法國） 其財政支持，Gérard Chatagnon 的量化 精漿中的蛋白質，凝膠電泳的 Véronique Solé，英語校正的 Maureen Collobert 和 Alain Sire 和 Patrice Papineau 的概念和實現 手套箱允許在受控的情況下使用 Langmuir 槽進行工作 大氣層。

附錄 A. 補充數據

 與本文相關的補充數據可以在 在線版本，見 //dx.doi.org/10.1016/j.colsurfb.2015.11。 034.

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應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——摘要、簡介

應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——材料與方法

應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——結果與討論

應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——結論、致謝！